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It is usually assumed that an action potential starts immediately at the stimulating electrode. if this were true, both methods for calculating conduction velocity would provide the same answer. when a strong stimulus intensity is used, the action potential may begin some distance away from the stimulating electrode. under these conditions, the difference method would be more accurate. did you observe any important difference between the conduction velocity values?

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Longer latency period due to slower conduction velocity (this is due to their smaller diameter and less myelination) Velocity = Distance / latency period

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