Answer:
- unequal light path lengths across the light beam
- unequal absorber concentration across the light beam
- changes in refractive index at high analyte concentration
- shifts in chemical equilibria as a function of concentration
- fluorescence of the sample, in which some of the absorbed light is re-emitted and strikes the detector
Explanation:
For a spectroscopic experiment to fulfill the Beer Lambert law, one requirement is that every photon of light must have an equal probability of absorption. Anything that disrupts these conditions leads to a deviation from the law.
The Beer Lambert law is given as follows:
A = Log (I₀/I)
One major source of deviation is stray light. This is any light that has a wave length outside the boundaries.
